Extractive Fermentation for Process integration and amplified pullulan production by A. pullulans in Aqueous Two Phase Systems
نویسندگان
چکیده
منابع مشابه
Production of pullulan by a thermotolerant aureobasidium pullulans strain in non-stirred fed batch fermentation process
Total 95 isolates of Aureobasidium pullulans were isolated from different flowers and leaves samples, out of which 11 thermotolerant strains produced pullulan. One thermotolerant non-melanin pullulan producing strain, designated as RG-5, produced highest pullulan (37.1±1.0 g/l) at 42(o)C, pH 5.5 in 48h of incubation with 3% sucrose and 0.5% ammonium sulphate in a non-stirred fed batch fermentor...
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A study was made to find out maximum partitioning of Bacillus licheniformis alkaline phosphatase in different ATPSs composed of different molecular weight of PEG X (X=2000, 4000, 6000) with salts (magnesium sulphate, sodium sulphate, sodium citrate) and polymers (dextran 40, dextran T500). Physicochemical factors such as effect of system pH, system temperature and production media were evaluate...
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Ten isolates of Aureobasidium pullulans were screened for the highest production of pullulan and minimal melanin production from Fayoum Governorate, Egypt. The optimum conditions for continuous culture were studied; temperature was 24°C, while pH 5.0, and agitation speed was at 500 rpm, while the aeration rate was 2.5 vvm and the best flow rate was 1 L/ 24 h. The maximum production of pullulan ...
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An extractive fermentation technique was developed using a thermoseparating reagent to form a two-phase system for simultaneous cell cultivation and downstream processing of extracellular Burkholderia cepacia lipase. A 10% (w/w) solution of ethylene oxide-propylene oxide (EOPO) with a molecular mass of 3900 g/mol and pH 8.5, a 200 rpm speed, and 30 °C were selected as the optimal conditions for...
متن کاملPullulan Elaboration by Aureobasidium pullulans Protoplasts.
Protoplasts of Aureobasidium pullulans are capable of producing pullulan. Biosynthesis of the polymer pullulan required induction with kinetics similar to those of whole cells. The protoplasts also produced a heteropolysaccharide component containing mannose, glucose, and galactose. The relative proportions of the pullulan and heteropolysaccharide fractions were a function of glucose concentrat...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2019
ISSN: 2045-2322
DOI: 10.1038/s41598-018-37314-y